Can adopting one’s morning routines influence employees’ experiences throughout the day? To answer this focal question, we examine the daily effects of a brief meditation in the morning on well-being throughout the day considering spillover effects from the home to the work domain and back. To identify the dominant underlying mechanisms of this daily spillover, we draw on the personality systems interactions theory that distinguishes between autonomous self-regulation and effortful self-control as two psychological processes that reflect the regulation of thoughts, emotions, and behaviors in alignment or contradiction with one’s interests, values, and goals. Accordingly, we hypothesized that meditating in the morning before work fosters autonomous self-regulation and reduces effortful self-control in the work domain, which subsequently facilitates the experience of flow at work and hence fosters subjective vitality in the home domain after work. A quasi-experimental daily-diary study over 10 days with a brief 10-min mindfulness intervention during the final 5 days with 78 participants (588 day-level data points) supported most of our predictions. More specifically, our data suggest a positive indirect effect of the intervention on subjective vitality in the evening via self-regulation and flow experience. However, there was no indirect effect of the intervention on subjective vitality via self-control. The results help to clarify how a mindfulness-based intervention can influence distinct regulatory processes and well-being, crossing boundaries between the work and home domains.